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The length, throughput and sequencing cost of both these technologies paint a pretty picture, and indeed they have proved useful in many settings, but sequencing accuracy is a problem with these technologies. The per-base sequencing accuracy has been estimated to be between 85% and 92% for PacBio sequencers and 87% to 98% for ONT machines56,61,62. This accuracy is much lower than either Sanger sequencing or Illumina reads. Characterizing, correcting and accounting for these errors is widely studied and it will be discussed in more detail in Sections 1.3 and 1.4.

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